Dialect internet celebrities represent a new type of digital cultural intermediary in the digital age. Their core function lies in transforming regional cultural genes into symbols recognizable, disseminated, and consumed by mobile media through a translation mechanism revealed by actor-network theory, thereby achieving cultural “symbolizability.” Against the backdrop of globalization and urbanization, which have triggered anxieties over cultural homogenization and endangered intergenerational transmission of dialects, this study analyzes 151 short videos and 925 user comments from 84 dialect internet celebrities using text analysis. Centered on Callon’s “four-stage translation model”, it systematically examines their cultural mediation practices. The research finds that the mediation practice of dialect internet celebrities is a dynamic network-building process driven by translation, connecting diverse and heterogeneous actors. At its heart is the dual mediating role of “linguistic signs” and “spatial relations.” Through strategies such as symbolic simplification, linguistic “memeification,” and aesthetic enhancement, these influencers translate regional culture into highly identifiable communication units. Non-human actors—including platform algorithms and physical environments—are mobilized alongside human actors such as fans and MCN agencies to co-construct dissemination pathways. Research shows that the mediating role of dialect-based internet celebrities lies in the tension between their agency and constraints—actively translating culture and constructing identity, yet deeply shaped by algorithmic rules and the logic of traffic-driven economics. Ultimately, this practice not only bridges geographical divides and reshapes cross-regional cultural identities, but also transforms online engagement into offline economic and social momentum through emotional mobilization, forming a closed loop from symbolic dissemination to value creation for regional cultures.